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parl flag ct wild type  (Addgene inc)


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    Addgene inc parl flag ct wild type
    Parl Flag Ct Wild Type, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+parl+flag+ct+wild+type/pcDNA3+PARL-FLAG-CT+wild+type+(Plasmid+%2313639)/pm40484322-141-0-6
    Average 93 stars, based on 8 article reviews
    parl flag ct wild type - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Spautin-1 promotes PINK1-PRKN-dependent mitophagy and improves associative learning capability in an alzheimer disease animal model
    Article Snippet: siRNA used in this study: siRNA targeting PINK1 (hs.Ri.PINK1.13), USP10 (hs.Ri.USP10.13), USP13 (hs.Ri.USP13.13), USP8 (hs.Ri.USP8.13) and USP15 (hs.Ri.USP15.13) were purchased from Integrated DNA Technologies ( https://www.idtdna.com/ ) Plasmids used in this study: pcDNA3 PARL-FLAG-CT wild type (Addgene 13,639) [ ] and pcDNA3 PARL-FLAG-CT S277G (Addgene 13,615) [ ] were gifts from Dr. Luca Pellegrini; LentiCRISPRv2 puro (Addgene 98,290) [ ] was a gift from Brett Stringer; pMD2.G (Addgene 12,259) and psPAX2 (Addgene 12,260) were gifts from Didier Trono. pcDNA-DEST47 PINK1 C-GFP (PINK1-C-GFP) [ ] was a gift from Mark Cookson.

    Plasmid Preparation:

    Article Title: Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury
    Article Snippet: Images were taken using Carl Zeiss Observer Z1 microscope 24 h after transfection. .. pcDNA3 PARL-FLAG-CT wild type (Addgene plasmid # 13639), pcDNA3 PARL-FLAG-CT S65A + T69A + S70A (Addgene plasmid # 13616), pcDNA3 PARL-FLAG-CT S65D + T69D + S70D (Addgene plasmid # 13617) were gifts from Luca Pellegrini [ , ]. ..



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    Image Search Results


    PGAM5 interferes with PINK1 processing independently of its phosphatase activity. A. PGAM5 mutant and isoform lacking phosphatase activity induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 wt, PGAM5 F244D or short PGAM5 isoform (isoform 2, UniProt #Q96HS1-2). ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. B. PGAM5 mutant lacking PARL/OMA1 cleaving site does not induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 or PGAM5 S24F. ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. C. TOMM7 overexpression increases the fraction of full-length PGAM5 in HEK293 cells expressing PGAM5-flag and TOMM7-myc. D. TOMM7 overexpression protects partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt or/and TOMM7. ****P < 0.0001, n = 9 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. E. OMA1 and PARL protect partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt and PARL or OMA1. ****P < 0.0001, n = 6 dishes, 20 fields per dish, One-way ANOVA followed by Sidak's multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Redox Biology

    Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

    doi: 10.1016/j.redox.2021.102186

    Figure Lengend Snippet: PGAM5 interferes with PINK1 processing independently of its phosphatase activity. A. PGAM5 mutant and isoform lacking phosphatase activity induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 wt, PGAM5 F244D or short PGAM5 isoform (isoform 2, UniProt #Q96HS1-2). ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. B. PGAM5 mutant lacking PARL/OMA1 cleaving site does not induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 or PGAM5 S24F. ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. C. TOMM7 overexpression increases the fraction of full-length PGAM5 in HEK293 cells expressing PGAM5-flag and TOMM7-myc. D. TOMM7 overexpression protects partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt or/and TOMM7. ****P < 0.0001, n = 9 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. E. OMA1 and PARL protect partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt and PARL or OMA1. ****P < 0.0001, n = 6 dishes, 20 fields per dish, One-way ANOVA followed by Sidak's multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

    Techniques: Activity Assay, Mutagenesis, Translocation Assay, Transfection, Over Expression, Expressing

    Journal: Redox Biology

    Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

    doi: 10.1016/j.redox.2021.102186

    Figure Lengend Snippet:

    Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

    Techniques: Recombinant, Lysis, Extraction, Blocking Assay, Isolation, DC Protein Assay, Caspase-Glo Assay, shRNA, Generated, Plasmid Preparation, Synthesized, Variant Assay